Isolation and Purification of Proteins (Biotechnology and Bioprocessing)
Rajni Hatti-Kaul, Bo Mattiasson
This publication details the isolation of proteins from biological materials, techniques for solid-liquid separation, concentration, crystallization, chromatography, scale-up, process monitoring, product formulation, and regulatory and commercial considerations in protein production. The authors discuss the release of protein from a biological host, selectivity in affinity chromatography, precipitation of proteins (both non-specific and specific), extraction for rapid protein isolation, adsorption as an initial step for the capture of proteins, scale-up and commercial production of recombinant proteins, and process monitoring in downstream processing.
Рік:
2003
Видання:
1
Видавництво:
CRC Press
Мова:
english
Сторінки:
676
ISBN 10:
0824707265
ISBN 13:
9780824707262
Файл:
PDF, 8.81 MB
IPFS:
,
english, 2003